Well, another one for the "don't put off until tomorrow what you can do today" camp.
About 15 minutes (including removing and replacing the strings) and a bit of 220 and the g and the e are much closer, all take off a bit more next time I change strings. Not much I can do about the b, but that's always the problem child. I think if I make another saddle from scratch I can get it a bit closer by basically leaving the edge flush, but as it is it's a bit rounded over, so I can't really add more compensation without also lowering the action.
I think part of my problem here was expecting the the Colosi saddle would be dead on.
Also, as for the twist...I can see a little something, if I look really closely, but don't feel it, and it doesn't affect playability. So...