It's always the B string for me! Especially on the 12 string, even when it's in tune it drives me nuts. I'm not sure why, but I always seem to think it's either out of tune, or not in tune with each other. I swear I adjust it every other song I play! Frustrating, but it seems worse some days than others. Might have something to do with the humidity?
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1957 Harmony Montery
2003 Guild JF30-12
2011 Epiphone EJ-160e
2011 Guild M120E
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